Bio rad protein ladders have been formulated and combined for use as molecular weight markers in a variety of applications including sds page ief 2 d page and western blotting.
High molecular weight ladder.
No need to heat reduce or add sample buffer prior to use.
Each for 133 00 add to cart 45 001 592.
The 2 d sds page standards have a pi range from 4 5 to 8 5 and a molecular weight range from 17 5 to 76 kd.
More intense bands on gels and blots and have improved band spacing for more accurate molecular weight determination.
Therefore when used in gel electrophoresis markers effectively provide a logarithmic scale by which to estimate the size of the other fragments.
Protein dna and rna marker.
A high molecular weight ladder polymer based on 5 5 6 6 tetrahydroxy 3 3 3 3 tetramethylspirobisindane and 1 4 dicyanotetraflurobenzene has been synthesized by polycondensation under high intensity mixing conditions at about 155 c and cyclic free products were obtained in high yield with low molecular weight distribution 1 7 2 3.
These protein ladders for sds page are available in three size ranges.
Low molecular weight range.
Color coded prestained protein marker high range 43 315 kda is a mixture of purified proteins covalently coupled to blue green orange or pink dyes that resolves to 8 bands between 43 and 315 kda when electrophoresed.
Generuler high range dna ladder can be labeled radioactively with t4 polynucleotide kinase see protocol in the resources.
Each marker is quantitated to yield uniform band intensities when stained with coomassie brilliant blue r 250 following sds page.
Neb dna ladders are available in standard formats and in a variety of convenient ready to load formats.
High intensity prestained multicolored bands allow distinction of closely related proteins.
Protein molecular weight marker ladder.
Thermo scientific generuler high range dna ladder is recommended for fast sizing and approximate quantification of high molecular weight double stranded dna on agarose gels in 1 5 hour 0 4 agarose gel.
44 3 200 kda and broad molecular weight range.
Neb also offers various conventional double stranded dna molecular weight markers with sizes up to 23 kb and markers for pulsed field gel electrophoresis pfge ranging from 1 5 to 1 018 kb.
Himark pre stained protein standard is designed for analysis of high molecular weight proteins and consists of nine protein bands ranging from 30 460 kda.
The ladder is a mixture of chromatography purified individual dna fragments.
Prestained protein ladders precision plus protein standards have the same gel migration patterns with three high intensity reference bands 25 50 and 75 kd and provide exceptional lot to lot molecular weight consistency.
6 5 200 kda.
A molecular weight size marker also referred to as a protein ladder dna ladder or rna ladder is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis using the principle that molecular weight is inversely proportional to migration rate through a gel matrix.
